Journal: EMBO Reports

Article Title: pH regulates hematopoietic stem cell potential via polyamines

doi: 10.15252/embr.202255373

Figure Lengend Snippet:

Article Snippet: anti‐rat DyLight488‐conjugated antibody , Jackson ImmunoResearch , Cat # 112‐485‐071.

Techniques: DNA Library Preparation, Modification, Recombinant, Software, Suspension

Journal: PLoS ONE

Article Title: CD36 Differently Regulates Macrophage Responses to Smooth and Rough Lipopolysaccharide

doi: 10.1371/journal.pone.0153558

Figure Lengend Snippet: (A) S-LPS at 1 μg/ml enhances binding of rCD36 to plate-adsorbed rCD14. (B) CD36-/- PEMs exhibit neither basal nor 1 μg/ml S-LPS-stimulated rCD14 binding. (C) S-LPS-stimulated binding of rCD14 to PEMs is blocked by anti-CD36 mAb, but not by anti-TLR2 or anti-TLR4/MD-2 mAb. (D) During 40 min co-incubation at 37°C, 200 ng/ml S-LPS stimulates much stronger rCD14 binding to PEMs than the same concentration of R-LPS. Graphs show means ± SEM of 3–4 replicates, obtained in single experiments, each representative of at least 3 similar experiments performed. Data on graphs C and D were analyzed with ANOVA, followed by the Dunnett’s post-test. *, p < 0.05; HRP-anti-Fc, HRP-conjugated F(ab’) 2 fragments of goat antibodies specific for Fc fragments of human IgG.

Article Snippet: Bound rCD36 was detected by 1-h incubation with 2 μg/ml of polyclonal goat anti-mouse CD36 Ab (R&D Systems), followed by 1-h incubation with 4 μg/ml of HRP-conjugated F(ab’) 2 fragments of donkey anti-goat IgG Ab (Rockland) in BSA-PBS.

Techniques: Binding Assay, Incubation, Concentration Assay

Journal: CEN Case Reports

Article Title: Membranous nephropathy with masked polyclonal IgG deposits associated with primary Sjögren’s syndrome

doi: 10.1007/s13730-020-00516-3

Figure Lengend Snippet: Additional staining of paraffin-embedded tissue treated with proteinase K digestion. The deposited IgG was polyclonal and amyloid P was not found in the glomeruli (a κ, b λ, c IgG, d serum amyloid P, e merged image of IgG and serum amyloid P staining)

Article Snippet: This time, we performed immunofluorescence using Dylight 488-conjugated polyclonal goat anti-human IgG antibody (NBP1-74980) purchased from Novus Biologicals (CO, USA), but again failed to reveal the deposition (data not shown).

Techniques: Staining